After deconvolution we run a program on our workstation that calculates a 3D reconstruction. There are basically two ways of rendering: volume rendering and surface rendering. Our volume rendering program is called FluVR; the surface rendering program is called Amira

 

Volume rendering. FluVR reconstructs, voxel for voxel, the fluorescence emitted from the inside of each of the little cubes of the 3D space.
 
Surface rendering: Amira constructs a triangular frame-surface around the object and covers it with a 'skin' of a reflecting material, the texture.
Example: volume 3D-rendered spiny dendrite of a neuron, green channel
Surface 3D-rendered same spiny dendrite of a neuron, green channel
Volume 3D-rendered spiny dendrite of a neuron, green channel plus red channel (fibers red; reconstructed integrated)
Surface 3D-rendered same spiny dendrite of a neuron, green channel plus red channel (fibers red; reconstructed integrated)
in imaging, touch is a little bit overlap!